Detailed information of TA system TA083812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239344..1239912
• Replicon: chromosome
• Accession: NZ_CP023608
• Organism: Mycobacterium tuberculosis strain LE410

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP464_RS06090
• Protein ID: WP_003405865.1
• Coordinates: 1239538..1239912 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP464_RS06085
• Protein ID: WP_003405863.1
• Coordinates: 1239344..1239541 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP464_RS06065	1235385..1236023	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP464_RS06070	1236113..1237120	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP464_RS06075	1237137..1238120	-	984	WP_003898731.1	hypothetical protein	-
CP464_RS06080	1238183..1239256	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP464_RS06085	1239344..1239541	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP464_RS06090	1239538..1239912	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP464_RS06095	1240115..1240813	+	699	WP_003898733.1	hypothetical protein	-
CP464_RS06100	1240931..1241116	+	186	WP_003901093.1	hypothetical protein	-
CP464_RS06105	1241043..1241318	-	276	WP_003405867.1	hypothetical protein	-
CP464_RS06110	1241561..1241884	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP464_RS06115	1241899..1242609	-	711	WP_003911399.1	hypothetical protein	-
CP464_RS21725	1242631..1242840	+	210	WP_003911400.1	hypothetical protein	-
CP464_RS06120	1242792..1243499	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T83812 WP_003405865.1 NZ_CP023608:1239538-1239912 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T83812 NZ_CP023608:1239538-1239912 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT83812 WP_003405863.1 NZ_CP023608:1239344-1239541 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT83812 NZ_CP023608:1239344-1239541 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07