Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2868356..2868987 Replicon chromosome
Accession NZ_CP023607
Organism Mycobacterium tuberculosis strain LE371

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag CP463_RS13950 Protein ID WP_003413174.1
Coordinates 2868610..2868987 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag CP463_RS13945 Protein ID WP_003413167.1
Coordinates 2868356..2868613 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP463_RS13910 2864270..2864584 + 315 WP_009937839.1 hypothetical protein -
CP463_RS13915 2864880..2866091 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CP463_RS13925 2866218..2866877 + 660 WP_003900846.1 LppA family lipoprotein -
CP463_RS13930 2866874..2867536 + 663 WP_003900848.1 hypothetical protein -
CP463_RS13935 2867533..2867811 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CP463_RS13940 2867904..2868317 + 414 WP_003413164.1 PIN domain nuclease -
CP463_RS13945 2868356..2868613 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
CP463_RS13950 2868610..2868987 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
CP463_RS13955 2869003..2869377 - 375 WP_003413177.1 hypothetical protein -
CP463_RS13960 2869477..2869872 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
CP463_RS13965 2869869..2870114 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
CP463_RS13975 2870525..2870944 - 420 WP_003413190.1 A24 family peptidase -
CP463_RS13980 2870956..2871765 - 810 WP_003413193.1 shikimate dehydrogenase -
CP463_RS13985 2871762..2873015 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CP463_RS13990 2873008..2873520 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T83774 WP_003413174.1 NZ_CP023607:2868610-2868987 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T83774 NZ_CP023607:2868610-2868987 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT83774 WP_003413167.1 NZ_CP023607:2868356-2868613 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT83774 NZ_CP023607:2868356-2868613 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References