Detailed information of TA system TA083751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239190..1239758
• Replicon: chromosome
• Accession: NZ_CP023607
• Organism: Mycobacterium tuberculosis strain LE371

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP463_RS06090
• Protein ID: WP_003405865.1
• Coordinates: 1239384..1239758 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP463_RS06085
• Protein ID: WP_003405863.1
• Coordinates: 1239190..1239387 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP463_RS06065	1235231..1235869	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP463_RS06070	1235959..1236966	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP463_RS06075	1236983..1237966	-	984	WP_003898731.1	hypothetical protein	-
CP463_RS06080	1238029..1239102	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP463_RS06085	1239190..1239387	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP463_RS06090	1239384..1239758	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP463_RS06095	1239961..1240659	+	699	WP_003898733.1	hypothetical protein	-
CP463_RS06100	1240777..1240962	+	186	WP_003901093.1	hypothetical protein	-
CP463_RS06105	1240889..1241164	-	276	WP_003405867.1	hypothetical protein	-
CP463_RS06110	1241407..1241730	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP463_RS06115	1241745..1242455	-	711	WP_003911399.1	hypothetical protein	-
CP463_RS21750	1242477..1242686	+	210	WP_003911400.1	hypothetical protein	-
CP463_RS06120	1242638..1243345	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T83751 WP_003405865.1 NZ_CP023607:1239384-1239758 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T83751 NZ_CP023607:1239384-1239758 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT83751 WP_003405863.1 NZ_CP023607:1239190-1239387 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT83751 NZ_CP023607:1239190-1239387 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07