Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110011..3110581 Replicon chromosome
Accession NZ_CP023606
Organism Mycobacterium tuberculosis strain LE103

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP462_RS15310 Protein ID WP_003414166.1
Coordinates 3110011..3110367 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP462_RS15315 Protein ID WP_003901465.1
Coordinates 3110351..3110581 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP462_RS15290 3105463..3107151 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP462_RS15295 3107155..3107481 - 327 WP_003414157.1 hypothetical protein -
CP462_RS15300 3107654..3108241 + 588 WP_023641719.1 DUF3558 family protein -
CP462_RS15305 3108260..3109909 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP462_RS15310 3110011..3110367 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP462_RS15315 3110351..3110581 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP462_RS15320 3110624..3111667 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CP462_RS15325 3111858..3112133 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP462_RS21535 3112309..3112563 - 255 WP_003917684.1 hypothetical protein -
CP462_RS15335 3112711..3113115 + 405 WP_009938577.1 hypothetical protein -
CP462_RS15340 3113112..3113303 + 192 WP_003414184.1 hypothetical protein -
CP462_RS15345 3113367..3114656 + 1290 Protein_2903 transposase family protein -
CP462_RS15350 3114890..3115147 + 258 WP_003899489.1 hypothetical protein -
CP462_RS15355 3115252..3115563 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T83719 WP_003414166.1 NZ_CP023606:c3110367-3110011 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T83719 NZ_CP023606:c3110367-3110011 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT83719 WP_003901465.1 NZ_CP023606:c3110581-3110351 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT83719 NZ_CP023606:c3110581-3110351 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References