Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3070014..3070693 Replicon chromosome
Accession NZ_CP023606
Organism Mycobacterium tuberculosis strain LE103

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag CP462_RS15085 Protein ID WP_003414059.1
Coordinates 3070014..3070430 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag CP462_RS15090 Protein ID WP_003414061.1
Coordinates 3070427..3070693 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP462_RS15065 3066066..3066968 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
CP462_RS15070 3067037..3067789 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
CP462_RS15075 3068033..3068308 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
CP462_RS15080 3068305..3069927 - 1623 WP_003906897.1 type I restriction-modification system subunit M -
CP462_RS15085 3070014..3070430 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
CP462_RS15090 3070427..3070693 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP462_RS15095 3070719..3071114 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
CP462_RS15100 3071111..3071380 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
CP462_RS15105 3071390..3072484 - 1095 WP_003414068.1 restriction endonuclease subunit S -
CP462_RS15110 3072481..3072900 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
CP462_RS15115 3072974..3073453 - 480 WP_003414073.1 dihydrofolate reductase -
CP462_RS15120 3073524..3074324 - 801 WP_009939104.1 thymidylate synthase -
CP462_RS15125 3074480..3075217 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T83717 WP_003414059.1 NZ_CP023606:c3070430-3070014 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T83717 NZ_CP023606:c3070430-3070014 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT83717 WP_003414061.1 NZ_CP023606:c3070693-3070427 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT83717 NZ_CP023606:c3070693-3070427 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References