Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1946896..1947509 Replicon chromosome
Accession NZ_CP023606
Organism Mycobacterium tuberculosis strain LE103

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag CP462_RS09375 Protein ID WP_003408465.1
Coordinates 1946896..1947285 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag CP462_RS09380 Protein ID WP_003408469.1
Coordinates 1947282..1947509 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP462_RS09345 1942526..1943440 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
CP462_RS09350 1943443..1944273 + 831 WP_003906661.1 cyclase family protein -
CP462_RS09355 1944273..1944622 + 350 Protein_1773 cupin domain-containing protein -
CP462_RS09360 1944675..1945493 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
CP462_RS09365 1945507..1946286 + 780 WP_003408460.1 IclR family transcriptional regulator -
CP462_RS09375 1946896..1947285 - 390 WP_003408465.1 PIN domain-containing protein Toxin
CP462_RS09380 1947282..1947509 - 228 WP_003408469.1 antitoxin Antitoxin
CP462_RS09385 1947727..1949211 + 1485 WP_009937637.1 biotin carboxylase -
CP462_RS09390 1949208..1950455 + 1248 WP_003408476.1 serine hydrolase -
CP462_RS09395 1950498..1950917 - 420 WP_003408483.1 hypothetical protein -
CP462_RS09400 1950907..1951617 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T83697 WP_003408465.1 NZ_CP023606:c1947285-1946896 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T83697 NZ_CP023606:c1947285-1946896 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT83697 WP_003408469.1 NZ_CP023606:c1947509-1947282 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT83697 NZ_CP023606:c1947509-1947282 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References