Detailed information of TA system TA083612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 640180..640856
• Replicon: chromosome
• Accession: NZ_CP023605
• Organism: Mycobacterium tuberculosis strain LE79

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: CP461_RS02940
• Protein ID: WP_003898500.1
• Coordinates: 640180..640593 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: CP461_RS02945
• Protein ID: WP_003402915.1
• Coordinates: 640590..640856 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP461_RS02910	635525..635827	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
CP461_RS02915	635887..636165	-	279	WP_003402901.1	hypothetical protein	-
CP461_RS02920	636162..637415	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
CP461_RS02925	637535..637921	-	387	WP_003402907.1	VOC family protein	-
CP461_RS02930	637984..638868	-	885	WP_003402909.1	SDR family oxidoreductase	-
CP461_RS02935	638964..639866	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
CP461_RS02940	640180..640593	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
CP461_RS02945	640590..640856	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
CP461_RS02950	641048..642763	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
CP461_RS02955	642841..644445	+	1605	WP_003402920.1	amidohydrolase	-
CP461_RS02960	644442..645422	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T83612 WP_003898500.1 NZ_CP023605:c640593-640180 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T83612 NZ_CP023605:c640593-640180 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT83612 WP_003402915.1 NZ_CP023605:c640856-640590 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT83612 NZ_CP023605:c640856-640590 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0