Detailed information of TA system TA083603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707360..3708034
• Replicon: chromosome
• Accession: NZ_CP023604
• Organism: Mycobacterium tuberculosis strain LE76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP460_RS18190
• Protein ID: WP_003417282.1
• Coordinates: 3707360..3707788 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP460_RS18195
• Protein ID: WP_003417286.1
• Coordinates: 3707792..3708034 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP460_RS18165	3703182..3703583	-	402	WP_003417264.1	cytidine deaminase	-
CP460_RS18170	3703724..3704158	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP460_RS18175	3704155..3704589	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP460_RS18180	3704718..3706490	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP460_RS18185	3706490..3707281	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP460_RS18190	3707360..3707788	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP460_RS18195	3707792..3708034	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP460_RS18200	3708156..3708770	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP460_RS18205	3708767..3709432	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP460_RS18210	3709433..3709966	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP460_RS21920	3709963..3710115	-	153	WP_003916129.1	hypothetical protein	-
CP460_RS18220	3710151..3711412	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T83603 WP_003417282.1 NZ_CP023604:c3707788-3707360 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T83603 NZ_CP023604:c3707788-3707360 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT83603 WP_003417286.1 NZ_CP023604:c3708034-3707792 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT83603 NZ_CP023604:c3708034-3707792 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59