Detailed information of TA system TA083542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707549..3708223
• Replicon: chromosome
• Accession: NZ_CP023603
• Organism: Mycobacterium tuberculosis strain LE63

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP459_RS18175
• Protein ID: WP_003417282.1
• Coordinates: 3707549..3707977 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP459_RS18180
• Protein ID: WP_003417286.1
• Coordinates: 3707981..3708223 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP459_RS18150	3703371..3703772	-	402	WP_003417264.1	cytidine deaminase	-
CP459_RS18155	3703913..3704347	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP459_RS18160	3704344..3704778	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP459_RS18165	3704907..3706679	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP459_RS18170	3706679..3707470	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP459_RS18175	3707549..3707977	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP459_RS18180	3707981..3708223	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP459_RS18185	3708345..3708959	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP459_RS18190	3708956..3709621	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP459_RS18195	3709622..3710155	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP459_RS21920	3710152..3710304	-	153	WP_003916129.1	hypothetical protein	-
CP459_RS18205	3710340..3711601	+	1262	WP_096868035.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T83542 WP_003417282.1 NZ_CP023603:c3707977-3707549 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T83542 NZ_CP023603:c3707977-3707549 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT83542 WP_003417286.1 NZ_CP023603:c3708223-3707981 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT83542 NZ_CP023603:c3708223-3707981 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59