Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2929992..2930706 Replicon chromosome
Accession NZ_CP023603
Organism Mycobacterium tuberculosis strain LE63

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag CP459_RS14250 Protein ID WP_003413460.1
Coordinates 2930266..2930706 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag CP459_RS14245 Protein ID WP_003413456.1
Coordinates 2929992..2930279 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP459_RS14210 2925414..2925659 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CP459_RS14215 2925656..2926060 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
CP459_RS14220 2926277..2926897 + 621 WP_003413441.1 DUF4178 domain-containing protein -
CP459_RS14225 2926908..2927402 + 495 WP_003413444.1 DUF2617 family protein -
CP459_RS14230 2927399..2927830 + 432 WP_003899390.1 DUF4247 domain-containing protein -
CP459_RS14235 2927855..2928313 + 459 WP_003413451.1 DUF350 domain-containing protein -
CP459_RS14240 2928310..2929881 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
CP459_RS14245 2929992..2930279 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
CP459_RS14250 2930266..2930706 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
CP459_RS14255 2930727..2931482 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
CP459_RS14260 2931615..2932211 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
CP459_RS14265 2932219..2933064 - 846 WP_003413466.1 acyl-CoA thioesterase II -
CP459_RS14270 2933093..2933992 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
CP459_RS14275 2934120..2934794 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T83532 WP_003413460.1 NZ_CP023603:2930266-2930706 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T83532 NZ_CP023603:2930266-2930706 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT83532 WP_003413456.1 NZ_CP023603:2929992-2930279 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT83532 NZ_CP023603:2929992-2930279 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References