Detailed information of TA system TA083507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239357..1239925
• Replicon: chromosome
• Accession: NZ_CP023603
• Organism: Mycobacterium tuberculosis strain LE63

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP459_RS06090
• Protein ID: WP_003405865.1
• Coordinates: 1239551..1239925 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP459_RS06085
• Protein ID: WP_003405863.1
• Coordinates: 1239357..1239554 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP459_RS06065	1235398..1236036	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP459_RS06070	1236126..1237133	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP459_RS06075	1237150..1238133	-	984	WP_003898731.1	hypothetical protein	-
CP459_RS06080	1238196..1239269	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP459_RS06085	1239357..1239554	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP459_RS06090	1239551..1239925	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP459_RS06095	1240128..1240826	+	699	WP_003898733.1	hypothetical protein	-
CP459_RS06100	1240944..1241129	+	186	WP_003901093.1	hypothetical protein	-
CP459_RS06105	1241056..1241331	-	276	WP_003405867.1	hypothetical protein	-
CP459_RS06110	1241574..1241897	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP459_RS06115	1241912..1242622	-	711	WP_003911399.1	hypothetical protein	-
CP459_RS21745	1242644..1242853	+	210	WP_003911400.1	hypothetical protein	-
CP459_RS06120	1242805..1243512	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T83507 WP_003405865.1 NZ_CP023603:1239551-1239925 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T83507 NZ_CP023603:1239551-1239925 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT83507 WP_003405863.1 NZ_CP023603:1239357-1239554 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT83507 NZ_CP023603:1239357-1239554 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07