Detailed information of TA system TA083500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 754965..755757
• Replicon: chromosome
• Accession: NZ_CP023603
• Organism: Mycobacterium tuberculosis strain LE63

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: CP459_RS03555
• Protein ID: WP_003403386.1
• Coordinates: 755320..755757 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: CP459_RS03550
• Protein ID: WP_003403381.1
• Coordinates: 754965..755210 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP459_RS03520	749985..751490	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
CP459_RS03525	751571..752581	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
CP459_RS03530	752969..753352	-	384	WP_070890539.1	VapC toxin family PIN domain ribonuclease	-
CP459_RS03535	753447..753602	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
CP459_RS03540	753678..754394	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
CP459_RS03545	754670..754978	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CP459_RS03550	754965..755210	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
CP459_RS03555	755320..755757	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP459_RS03560	755754..756008	-	255	WP_003911263.1	antitoxin VapB7	-
CP459_RS03565	756122..758485	+	2364	WP_003898547.1	arylsulfatase AtsD	-
CP459_RS03570	758548..758874	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CP459_RS03575	758786..759124	+	339	WP_003403405.1	PIN domain-containing protein	-
CP459_RS03580	759121..759294	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T83500 WP_003403386.1 NZ_CP023603:c755757-755320 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T83500 NZ_CP023603:c755757-755320 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT83500 WP_003403381.1 NZ_CP023603:c755210-754965 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT83500 NZ_CP023603:c755210-754965 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0