Detailed information of TA system TA083112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136535..3137139
• Replicon: chromosome
• Accession: NZ_CP023596
• Organism: Mycobacterium tuberculosis strain SLM063

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: CP452_RS15455
• Protein ID: WP_003414492.1
• Coordinates: 3136535..3136927 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: CP452_RS15460
• Protein ID: WP_003414495.1
• Coordinates: 3136924..3137139 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP452_RS15425	3131685..3132473	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
CP452_RS15430	3132807..3133352	-	546	WP_003913758.1	DUF1802 family protein	-
CP452_RS15435	3133624..3134508	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CP452_RS15440	3134511..3135398	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
CP452_RS15445	3135703..3136248	-	546	WP_003899500.1	DUF1802 family protein	-
CP452_RS15450	3136245..3136514	-	270	WP_003912856.1	DUF2277 family protein	-
CP452_RS15455	3136535..3136927	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP452_RS15460	3136924..3137139	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CP452_RS15465	3137186..3137935	+	750	WP_003414497.1	enoyl-CoA hydratase	-
CP452_RS15470	3138014..3139096	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
CP452_RS15475	3139089..3140399	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
CP452_RS15480	3140402..3141229	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
CP452_RS15485	3141226..3142137	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T83112 WP_003414492.1 NZ_CP023596:c3136927-3136535 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T83112 NZ_CP023596:c3136927-3136535 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT83112 WP_003414495.1 NZ_CP023596:c3137139-3136924 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT83112 NZ_CP023596:c3137139-3136924 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8