Detailed information of TA system TA083081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239326..1239894
• Replicon: chromosome
• Accession: NZ_CP023596
• Organism: Mycobacterium tuberculosis strain SLM063

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP452_RS06090
• Protein ID: WP_003405865.1
• Coordinates: 1239520..1239894 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP452_RS06085
• Protein ID: WP_003405863.1
• Coordinates: 1239326..1239523 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP452_RS06065	1235367..1236005	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP452_RS06070	1236095..1237102	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP452_RS06075	1237119..1238102	-	984	WP_003898731.1	hypothetical protein	-
CP452_RS06080	1238165..1239238	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP452_RS06085	1239326..1239523	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP452_RS06090	1239520..1239894	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP452_RS06095	1240097..1240795	+	699	WP_003898733.1	hypothetical protein	-
CP452_RS06100	1240913..1241098	+	186	WP_003901093.1	hypothetical protein	-
CP452_RS06105	1241025..1241300	-	276	WP_003405867.1	hypothetical protein	-
CP452_RS06110	1241543..1241866	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP452_RS06115	1241881..1242591	-	711	WP_003911399.1	hypothetical protein	-
CP452_RS21705	1242613..1242822	+	210	WP_003911400.1	hypothetical protein	-
CP452_RS06120	1242774..1243481	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T83081 WP_003405865.1 NZ_CP023596:1239520-1239894 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T83081 NZ_CP023596:1239520-1239894 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT83081 WP_003405863.1 NZ_CP023596:1239326-1239523 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT83081 NZ_CP023596:1239326-1239523 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07