Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2234029..2234615 Replicon chromosome
Accession NZ_CP023595
Organism Mycobacterium tuberculosis strain SLM060

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag CP451_RS10845 Protein ID WP_003410010.1
Coordinates 2234029..2234373 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag CP451_RS10850 Protein ID WP_003410014.1
Coordinates 2234367..2234615 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP451_RS10805 2229735..2230334 + 600 WP_003910883.1 L-lysine exporter -
CP451_RS10810 2230750..2231178 + 429 WP_003409992.1 cellulose-binding protein -
CP451_RS10815 2231404..2231943 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
CP451_RS10825 2232463..2233023 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
CP451_RS10830 2233020..2233361 - 342 WP_003410003.1 DUF2384 domain-containing protein -
CP451_RS10835 2233447..2233704 + 258 WP_003410006.1 hypothetical protein -
CP451_RS10840 2233605..2233940 - 336 WP_003410009.1 dehydrogenase -
CP451_RS10845 2234029..2234373 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
CP451_RS10850 2234367..2234615 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
CP451_RS10855 2234715..2237030 - 2316 WP_003899120.1 cation transporter ATPase CptG -
CP451_RS10860 2237027..2237299 - 273 WP_003410017.1 DUF1490 family protein -
CP451_RS10865 2237352..2237708 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
CP451_RS10870 2237865..2238632 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T83034 WP_003410010.1 NZ_CP023595:c2234373-2234029 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T83034 NZ_CP023595:c2234373-2234029 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT83034 WP_003410014.1 NZ_CP023595:c2234615-2234367 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT83034 NZ_CP023595:c2234615-2234367 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References