Detailed information of TA system TA083008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 703152..703795
• Replicon: chromosome
• Accession: NZ_CP023595
• Organism: Mycobacterium tuberculosis strain SLM060

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: CP451_RS03250
• Protein ID: WP_003403187.1
• Coordinates: 703394..703795 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP451_RS03245
• Protein ID: WP_070891125.1
• Coordinates: 703152..703397 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP451_RS03210	698432..698962	-	531	WP_003917320.1	two component system sensor kinase HK2	-
CP451_RS03215	698946..699641	-	696	WP_003918099.1	two-component system response regulator TcrA	-
CP451_RS03220	699764..700075	+	312	WP_003403164.1	hypothetical protein	-
CP451_RS03225	700147..701097	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
CP451_RS03230	701338..701922	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
CP451_RS03235	701924..702634	+	711	Protein_626	transposase	-
CP451_RS03240	702637..703107	+	471	WP_003898523.1	hypothetical protein	-
CP451_RS03245	703152..703397	+	246	WP_070891125.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP451_RS03250	703394..703795	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP451_RS21460	704183..704350	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
CP451_RS21465	704383..704580	-	198	WP_003403191.1	hypothetical protein	-
CP451_RS03270	704660..705817	-	1158	WP_003898524.1	hypothetical protein	-
CP451_RS03275	705869..706090	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
CP451_RS03280	706232..706837	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T83008 WP_003403187.1 NZ_CP023595:703394-703795 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T83008 NZ_CP023595:703394-703795 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8841.17 Da
• Isoelectric Point: 10.3486

>AT83008 WP_070891125.1 NZ_CP023595:703152-703397 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLVRLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT83008 NZ_CP023595:703152-703397 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGTCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6