Detailed information of TA system TA082981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2854178..2854818
• Replicon: chromosome
• Accession: NZ_CP023594
• Organism: Mycobacterium tuberculosis strain SLM056

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: CP450_RS13840
• Protein ID: WP_003412970.1
• Coordinates: 2854178..2854597 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: CP450_RS13845
• Protein ID: WP_003412975.1
• Coordinates: 2854594..2854818 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP450_RS13810	2849763..2850485	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
CP450_RS13820	2851002..2851229	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
CP450_RS13825	2851226..2851627	+	402	WP_003412963.1	PIN domain-containing protein	-
CP450_RS13830	2851662..2852582	-	921	WP_003412965.1	restriction endonuclease	-
CP450_RS21850	2852923..2853168	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CP450_RS13835	2853227..2854177	+	951	WP_003911916.1	Lsr2 family protein	-
CP450_RS13840	2854178..2854597	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP450_RS13845	2854594..2854818	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
CP450_RS13850	2854849..2857692	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
CP450_RS13855	2857764..2858165	-	402	WP_003412981.1	hypothetical protein	-
CP450_RS13860	2858165..2858635	-	471	WP_003899364.1	transcription antitermination factor NusB	-
CP450_RS13865	2858638..2859201	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T82981 WP_003412970.1 NZ_CP023594:c2854597-2854178 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T82981 NZ_CP023594:c2854597-2854178 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT82981 WP_003412975.1 NZ_CP023594:c2854818-2854594 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT82981 NZ_CP023594:c2854818-2854594 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09