Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225360..2226048 Replicon chromosome
Accession NZ_CP023593
Organism Mycobacterium tuberculosis strain SLM040

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag CP449_RS10775 Protein ID WP_003899117.1
Coordinates 2225360..2225779 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP449_RS10780 Protein ID WP_003409968.1
Coordinates 2225788..2226048 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP449_RS10755 2220855..2221703 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP449_RS10760 2221666..2223111 - 1446 WP_003899115.1 APC family permease -
CP449_RS10765 2223290..2223976 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
CP449_RS10770 2224167..2225135 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP449_RS10775 2225360..2225779 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
CP449_RS10780 2225788..2226048 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP449_RS10785 2226191..2227867 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP449_RS10790 2227855..2228508 - 654 WP_003409976.1 cutinase Cfp21 -
CP449_RS10795 2228623..2228853 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP449_RS10800 2228938..2229849 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP449_RS10805 2229958..2230557 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T82911 WP_003899117.1 NZ_CP023593:c2225779-2225360 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T82911 NZ_CP023593:c2225779-2225360 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCATGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT82911 WP_003409968.1 NZ_CP023593:c2226048-2225788 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT82911 NZ_CP023593:c2226048-2225788 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References