Detailed information of TA system TA082798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2807979..2808631
• Replicon: chromosome
• Accession: NZ_CP023591
• Organism: Mycobacterium tuberculosis strain TBV5365

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP447_RS13620
• Protein ID: WP_096872239.1
• Coordinates: 2808206..2808631 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ24
• Locus tag: CP447_RS13615
• Protein ID: WP_003412749.1
• Coordinates: 2807979..2808200 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP447_RS13600	2806219..2806425	-	207	WP_003899347.1	hypothetical protein	-
CP447_RS13605	2806561..2807184	+	624	WP_003900858.1	TIGR00725 family protein	-
CP447_RS13610	2807174..2807926	+	753	WP_003900859.1	hypothetical protein	-
CP447_RS13615	2807979..2808200	+	222	WP_003412749.1	antitoxin	Antitoxin
CP447_RS13620	2808206..2808631	+	426	WP_096872239.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP447_RS13625	2808654..2809835	-	1182	WP_003899351.1	2-oxo acid dehydrogenase subunit E2	-
CP447_RS13630	2809832..2810878	-	1047	WP_003412757.1	3-methyl-2-oxobutanoate dehydrogenase subunit beta	-
CP447_RS13635	2810889..2811992	-	1104	WP_003412761.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
CP447_RS13640	2812251..2813072	-	822	WP_003412766.1	citrate (pro-3S)-lyase subunit beta	-
CP447_RS13645	2813069..2813626	-	558	WP_003412768.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-139)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15333.82 Da
• Isoelectric Point: 8.1220

>T82798 WP_096872239.1 NZ_CP023591:2808206-2808631 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARPVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Nucleotide

• Length: 426 bp

>T82798 NZ_CP023591:2808206-2808631 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACCGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7864.93 Da
• Isoelectric Point: 4.5258

>AT82798 WP_003412749.1 NZ_CP023591:2807979-2808200 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT82798 NZ_CP023591:2807979-2808200 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW03