Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2194554..2195257 Replicon chromosome
Accession NZ_CP023591
Organism Mycobacterium tuberculosis strain TBV5365

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag CP447_RS10545 Protein ID WP_003409778.1
Coordinates 2194554..2194883 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag CP447_RS10550 Protein ID WP_003409780.1
Coordinates 2194880..2195257 (-) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP447_RS10525 2190937..2192007 + 1071 WP_003899091.1 epoxide hydrolase EphB -
CP447_RS10530 2192004..2192519 + 516 WP_003409718.1 flavin reductase family protein -
CP447_RS10535 2192516..2193577 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
CP447_RS10540 2193574..2194344 + 771 WP_003409775.1 SDR family oxidoreductase -
CP447_RS10545 2194554..2194883 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CP447_RS10550 2194880..2195257 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
CP447_RS10555 2195254..2195844 - 591 WP_003409784.1 SEC-C domain-containing protein -
CP447_RS10560 2195899..2197263 + 1365 WP_096872155.1 HNH endonuclease -
CP447_RS10565 2197418..2197870 - 453 WP_003899095.1 lipoprotein -
CP447_RS10570 2197934..2198335 + 402 WP_003409869.1 hypothetical protein -
CP447_RS10575 2198328..2198510 - 183 WP_003409870.1 hypothetical protein -
CP447_RS10580 2198624..2198974 - 351 WP_003899096.1 hypothetical protein -
CP447_RS10585 2198985..2199887 - 903 WP_003899097.1 hypothetical protein -
CP447_RS10590 2199908..2200099 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-103)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T82787 WP_003409778.1 NZ_CP023591:c2194883-2194554 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T82787 NZ_CP023591:c2194883-2194554 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT82787 WP_003409780.1 NZ_CP023591:c2195257-2194880 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT82787 NZ_CP023591:c2195257-2194880 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TLK7


Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References