Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 754914..755706 Replicon chromosome
Accession NZ_CP023591
Organism Mycobacterium tuberculosis strain TBV5365

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag CP447_RS03550 Protein ID WP_003403386.1
Coordinates 755269..755706 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag CP447_RS03545 Protein ID WP_003403381.1
Coordinates 754914..755159 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP447_RS03515 749934..751439 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP447_RS03520 751520..752530 + 1011 WP_198403350.1 ABC transporter ATP-binding protein -
CP447_RS03525 752918..753301 - 384 WP_003403365.1 ribonuclease VapC6 -
CP447_RS03530 753396..753551 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
CP447_RS03535 753627..754343 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP447_RS03540 754619..754927 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
CP447_RS03545 754914..755159 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
CP447_RS03550 755269..755706 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
CP447_RS03555 755703..755957 - 255 WP_003911263.1 antitoxin VapB7 -
CP447_RS03560 756071..758434 + 2364 WP_003898547.1 arylsulfatase AtsD -
CP447_RS03565 758497..758823 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP447_RS03570 758735..759073 + 339 WP_003403405.1 PIN domain-containing protein -
CP447_RS03575 759070..759243 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T82771 WP_003403386.1 NZ_CP023591:c755706-755269 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T82771 NZ_CP023591:c755706-755269 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT82771 WP_003403381.1 NZ_CP023591:c755159-754914 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT82771 NZ_CP023591:c755159-754914 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References