Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109986..3110556 Replicon chromosome
Accession NZ_CP023590
Organism Mycobacterium tuberculosis strain TBV5362

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP446_RS15320 Protein ID WP_003414166.1
Coordinates 3109986..3110342 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP446_RS15325 Protein ID WP_003901465.1
Coordinates 3110326..3110556 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP446_RS15300 3105438..3107126 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP446_RS15305 3107130..3107456 - 327 WP_003414157.1 hypothetical protein -
CP446_RS15310 3107629..3108216 + 588 WP_003914429.1 DUF3558 family protein -
CP446_RS15315 3108235..3109884 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP446_RS15320 3109986..3110342 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP446_RS15325 3110326..3110556 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP446_RS15330 3110599..3111642 - 1044 WP_031697622.1 DUF2293 domain-containing protein -
CP446_RS15335 3111833..3112108 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP446_RS21550 3112284..3112538 - 255 WP_003917684.1 hypothetical protein -
CP446_RS15345 3112686..3113090 + 405 WP_009938577.1 hypothetical protein -
CP446_RS15350 3113087..3113278 + 192 WP_003414184.1 hypothetical protein -
CP446_RS15355 3113342..3114631 + 1290 Protein_2902 transposase family protein -
CP446_RS15360 3114865..3115122 + 258 WP_003899489.1 hypothetical protein -
CP446_RS15365 3115227..3115538 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T82746 WP_003414166.1 NZ_CP023590:c3110342-3109986 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T82746 NZ_CP023590:c3110342-3109986 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT82746 WP_003901465.1 NZ_CP023590:c3110556-3110326 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT82746 NZ_CP023590:c3110556-3110326 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References