Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230546..1231177 Replicon chromosome
Accession NZ_CP023590
Organism Mycobacterium tuberculosis strain TBV5362

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag CP446_RS06040 Protein ID WP_003898728.1
Coordinates 1230546..1230857 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag CP446_RS06045 Protein ID WP_003405836.1
Coordinates 1230857..1231177 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP446_RS06020 1226027..1227451 - 1425 WP_003405805.1 class II fumarate hydratase -
CP446_RS06025 1227482..1228570 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
CP446_RS06030 1228626..1229270 + 645 WP_003915494.1 DUF4245 domain-containing protein -
CP446_RS06035 1229277..1230434 - 1158 WP_003898727.1 AI-2E family transporter -
CP446_RS06040 1230546..1230857 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
CP446_RS06045 1230857..1231177 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
CP446_RS06050 1231187..1232712 + 1526 Protein_1148 carboxylesterase/lipase family protein -
CP446_RS06055 1232639..1233829 - 1191 WP_176748187.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
CP446_RS06060 1233851..1234108 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
CP446_RS06065 1234098..1235345 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
CP446_RS06070 1235342..1235980 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T82716 WP_003898728.1 NZ_CP023590:c1230857-1230546 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T82716 NZ_CP023590:c1230857-1230546 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT82716 WP_003405836.1 NZ_CP023590:c1231177-1230857 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT82716 NZ_CP023590:c1231177-1230857 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References