Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109959..3110529 Replicon chromosome
Accession NZ_CP023589
Organism Mycobacterium tuberculosis strain TBV5000

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP445_RS15315 Protein ID WP_003414166.1
Coordinates 3109959..3110315 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP445_RS15320 Protein ID WP_003901465.1
Coordinates 3110299..3110529 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP445_RS15295 3105411..3107099 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP445_RS15300 3107103..3107429 - 327 WP_003414157.1 hypothetical protein -
CP445_RS15305 3107602..3108189 + 588 WP_003914429.1 DUF3558 family protein -
CP445_RS15310 3108208..3109857 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CP445_RS15315 3109959..3110315 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP445_RS15320 3110299..3110529 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP445_RS15325 3110572..3111615 - 1044 WP_031697622.1 DUF2293 domain-containing protein -
CP445_RS15330 3111806..3112081 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP445_RS21535 3112257..3112511 - 255 WP_003917684.1 hypothetical protein -
CP445_RS15340 3112659..3113063 + 405 WP_009938577.1 hypothetical protein -
CP445_RS15345 3113060..3113251 + 192 WP_003414184.1 hypothetical protein -
CP445_RS15350 3113315..3114604 + 1290 Protein_2901 transposase family protein -
CP445_RS15355 3114838..3115095 + 258 WP_003899489.1 hypothetical protein -
CP445_RS15360 3115200..3115511 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T82685 WP_003414166.1 NZ_CP023589:c3110315-3109959 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T82685 NZ_CP023589:c3110315-3109959 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT82685 WP_003901465.1 NZ_CP023589:c3110529-3110299 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT82685 NZ_CP023589:c3110529-3110299 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References