Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225138..2225826 Replicon chromosome
Accession NZ_CP023588
Organism Mycobacterium tuberculosis strain TBDM425

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag CP444_RS10775 Protein ID WP_003409958.1
Coordinates 2225138..2225557 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP444_RS10780 Protein ID WP_003409968.1
Coordinates 2225566..2225826 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP444_RS10755 2220633..2221481 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP444_RS10760 2221444..2222889 - 1446 WP_003899115.1 APC family permease -
CP444_RS10765 2223068..2223754 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
CP444_RS10770 2223945..2224913 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP444_RS10775 2225138..2225557 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
CP444_RS10780 2225566..2225826 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP444_RS10785 2225969..2227645 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP444_RS10790 2227633..2228286 - 654 WP_003409976.1 cutinase Cfp21 -
CP444_RS10795 2228401..2228631 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP444_RS10800 2228716..2229627 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP444_RS10805 2229736..2230335 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T82607 WP_003409958.1 NZ_CP023588:c2225557-2225138 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T82607 NZ_CP023588:c2225557-2225138 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT82607 WP_003409968.1 NZ_CP023588:c2225826-2225566 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT82607 NZ_CP023588:c2225826-2225566 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References