Detailed information of TA system TA082604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194368..2195071
• Replicon: chromosome
• Accession: NZ_CP023588
• Organism: Mycobacterium tuberculosis strain TBDM425

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CP444_RS10555
• Protein ID: WP_003409778.1
• Coordinates: 2194368..2194697 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CP444_RS10560
• Protein ID: WP_003409780.1
• Coordinates: 2194694..2195071 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP444_RS10535	2190751..2191821	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CP444_RS10540	2191818..2192333	+	516	WP_003409718.1	flavin reductase family protein	-
CP444_RS10545	2192330..2193391	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CP444_RS10550	2193388..2194158	+	771	WP_003409775.1	SDR family oxidoreductase	-
CP444_RS10555	2194368..2194697	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CP444_RS10560	2194694..2195071	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CP444_RS10565	2195068..2195658	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CP444_RS10570	2195713..2197077	+	1365	WP_014585532.1	HNH endonuclease	-
CP444_RS10575	2197232..2197684	-	453	WP_070899953.1	hypothetical protein	-
CP444_RS10580	2197748..2198149	+	402	WP_003409869.1	hypothetical protein	-
CP444_RS10585	2198142..2198324	-	183	WP_003409870.1	hypothetical protein	-
CP444_RS10590	2198438..2198788	-	351	WP_003899096.1	hypothetical protein	-
CP444_RS10595	2198799..2199701	-	903	WP_003899097.1	hypothetical protein	-
CP444_RS10600	2199722..2199913	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T82604 WP_003409778.1 NZ_CP023588:c2194697-2194368 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T82604 NZ_CP023588:c2194697-2194368 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT82604 WP_003409780.1 NZ_CP023588:c2195071-2194694 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT82604 NZ_CP023588:c2195071-2194694 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8