Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 716197..716846 Replicon chromosome
Accession NZ_CP023588
Organism Mycobacterium tuberculosis strain TBDM425

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag CP444_RS03345 Protein ID WP_003403236.1
Coordinates 716451..716846 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag CP444_RS03340 Protein ID WP_003403235.1
Coordinates 716197..716451 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP444_RS21705 711323..712018 + 696 WP_003900189.1 galactose-1-phosphate uridylyltransferase -
CP444_RS21710 712015..712506 + 492 WP_012054156.1 galactose-1-phosphate uridylyltransferase -
CP444_RS03320 712503..713594 + 1092 WP_003906403.1 galactokinase -
CP444_RS03330 713989..715053 + 1065 WP_003898532.1 hypothetical protein -
CP444_RS03335 715157..716104 + 948 WP_003403232.1 DUF732 domain-containing protein -
CP444_RS03340 716197..716451 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
CP444_RS03345 716451..716846 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
CP444_RS03350 716940..717680 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
CP444_RS03355 717812..718072 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP444_RS03360 718069..718476 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
CP444_RS03365 718548..719699 - 1152 WP_003403248.1 FIST C-terminal domain-containing protein -
CP444_RS03370 719792..721519 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T82584 WP_003403236.1 NZ_CP023588:716451-716846 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T82584 NZ_CP023588:716451-716846 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT82584 WP_003403235.1 NZ_CP023588:716197-716451 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT82584 NZ_CP023588:716197-716451 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References