Detailed information of TA system TA082569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707374..3708048
• Replicon: chromosome
• Accession: NZ_CP023587
• Organism: Mycobacterium tuberculosis strain ME1473

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP443_RS18190
• Protein ID: WP_003417282.1
• Coordinates: 3707374..3707802 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP443_RS18195
• Protein ID: WP_003417286.1
• Coordinates: 3707806..3708048 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP443_RS18165	3703196..3703597	-	402	WP_003417264.1	cytidine deaminase	-
CP443_RS18170	3703738..3704172	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP443_RS18175	3704169..3704603	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP443_RS18180	3704732..3706504	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP443_RS18185	3706504..3707295	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP443_RS18190	3707374..3707802	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP443_RS18195	3707806..3708048	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP443_RS18200	3708170..3708784	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP443_RS18205	3708781..3709446	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP443_RS18210	3709447..3709980	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP443_RS21955	3709977..3710129	-	153	WP_003916129.1	hypothetical protein	-
CP443_RS18220	3710165..3711426	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T82569 WP_003417282.1 NZ_CP023587:c3707802-3707374 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T82569 NZ_CP023587:c3707802-3707374 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT82569 WP_003417286.1 NZ_CP023587:c3708048-3707806 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT82569 NZ_CP023587:c3708048-3707806 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59