Detailed information of TA system TA082565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136369..3136973
• Replicon: chromosome
• Accession: NZ_CP023587
• Organism: Mycobacterium tuberculosis strain ME1473

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: CP443_RS15475
• Protein ID: WP_003414492.1
• Coordinates: 3136369..3136761 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: CP443_RS15480
• Protein ID: WP_003414495.1
• Coordinates: 3136758..3136973 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP443_RS15445	3131519..3132307	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
CP443_RS15450	3132641..3133186	-	546	WP_003913758.1	DUF1802 family protein	-
CP443_RS15455	3133458..3134342	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CP443_RS15460	3134345..3135232	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
CP443_RS15465	3135537..3136082	-	546	WP_003899500.1	DUF1802 family protein	-
CP443_RS15470	3136079..3136348	-	270	WP_003414489.1	DUF2277 family protein	-
CP443_RS15475	3136369..3136761	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP443_RS15480	3136758..3136973	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CP443_RS15485	3137020..3137769	+	750	WP_003414497.1	enoyl-CoA hydratase	-
CP443_RS15490	3137848..3138930	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
CP443_RS15495	3138923..3140233	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
CP443_RS15500	3140236..3141063	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
CP443_RS15505	3141060..3141971	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T82565 WP_003414492.1 NZ_CP023587:c3136761-3136369 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T82565 NZ_CP023587:c3136761-3136369 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT82565 WP_003414495.1 NZ_CP023587:c3136973-3136758 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT82565 NZ_CP023587:c3136973-3136758 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8