Detailed information of TA system TA082507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3182819..3183506
• Replicon: chromosome
• Accession: NZ_CP023586
• Organism: Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: CP442_RS15680
• Protein ID: WP_003414624.1
• Coordinates: 3183063..3183506 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: CP442_RS15675
• Protein ID: WP_003414620.1
• Coordinates: 3182819..3183076 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP442_RS15655	3178139..3178993	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
CP442_RS15660	3179049..3180212	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
CP442_RS15665	3180229..3181443	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
CP442_RS15670	3181451..3182692	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
CP442_RS15675	3182819..3183076	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP442_RS15680	3183063..3183506	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP442_RS15685	3183586..3184248	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
CP442_RS15690	3184344..3184535	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CP442_RS15695	3184867..3186615	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
CP442_RS15700	3186711..3187292	+	582	WP_003414644.1	fasciclin domain-containing protein	-
CP442_RS15705	3187392..3187658	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T82507 WP_003414624.1 NZ_CP023586:3183063-3183506 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T82507 NZ_CP023586:3183063-3183506 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT82507 WP_003414620.1 NZ_CP023586:3182819-3183076 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT82507 NZ_CP023586:3182819-3183076 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0