Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2807771..2808423 Replicon chromosome
Accession NZ_CP023586
Organism Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag CP442_RS13625 Protein ID WP_003905869.1
Coordinates 2807998..2808423 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag CP442_RS13620 Protein ID WP_003412749.1
Coordinates 2807771..2807992 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP442_RS13605 2806011..2806217 - 207 WP_003899347.1 hypothetical protein -
CP442_RS13610 2806353..2806976 + 624 WP_003900858.1 TIGR00725 family protein -
CP442_RS13615 2806966..2807718 + 753 WP_003901416.1 hypothetical protein -
CP442_RS13620 2807771..2807992 + 222 WP_003412749.1 antitoxin Antitoxin
CP442_RS13625 2807998..2808423 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
CP442_RS13630 2808446..2809627 - 1182 WP_003905870.1 2-oxo acid dehydrogenase subunit E2 -
CP442_RS13635 2809624..2810670 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
CP442_RS13640 2810681..2811784 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
CP442_RS13645 2812043..2812864 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
CP442_RS13650 2812861..2813418 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T82494 WP_003905869.1 NZ_CP023586:2807998-2808423 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T82494 NZ_CP023586:2807998-2808423 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT82494 WP_003412749.1 NZ_CP023586:2807771-2807992 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT82494 NZ_CP023586:2807771-2807992 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References