Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 840849..841558 Replicon chromosome
Accession NZ_CP023586
Organism Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)


Gene name vapC Uniprot ID P9WF74
Locus tag CP442_RS04050 Protein ID WP_003403837.1
Coordinates 841130..841558 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TEX4
Locus tag CP442_RS04045 Protein ID WP_003403834.1
Coordinates 840849..841106 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP442_RS04040 838353..840758 + 2406 WP_096865969.1 PE family protein -
CP442_RS04045 840849..841106 + 258 WP_003403834.1 ribbon-helix-helix protein, CopG family Antitoxin
CP442_RS04050 841130..841558 + 429 WP_003403837.1 type II toxin-antitoxin system VapC family toxin Toxin
CP442_RS04055 841648..841822 - 175 Protein_780 transposase -
CP442_RS04060 841935..842180 + 246 WP_003403841.1 hypothetical protein -
CP442_RS04065 842249..843133 - 885 WP_003403844.1 3-hydroxyisobutyrate dehydrogenase -
CP442_RS04070 843144..844316 - 1173 WP_003403845.1 acyl-CoA dehydrogenase FadE9 -
CP442_RS04075 844323..845855 - 1533 WP_003403847.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-36)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15831.27 Da        Isoelectric Point: 7.5536

>T82470 WP_003403837.1 NZ_CP023586:841130-841558 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Download         Length: 429 bp

>T82470 NZ_CP023586:841130-841558 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9114.28 Da        Isoelectric Point: 10.6530

>AT82470 WP_003403834.1 NZ_CP023586:840849-841106 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Download         Length: 258 bp

>AT82470 NZ_CP023586:840849-841106 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDR3

Antitoxin

Source ID Structure
AlphaFold DB G0TEX4

References