Detailed information of TA system TA082462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 703154..703797
• Replicon: chromosome
• Accession: NZ_CP023586
• Organism: Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: CP442_RS03240
• Protein ID: WP_003403187.1
• Coordinates: 703396..703797 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP442_RS03235
• Protein ID: WP_070891125.1
• Coordinates: 703154..703399 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP442_RS03200	698434..698964	-	531	WP_003917320.1	two component system sensor kinase HK2	-
CP442_RS03205	698948..699643	-	696	WP_003918099.1	two-component system response regulator TcrA	-
CP442_RS03210	699766..700077	+	312	WP_003403164.1	hypothetical protein	-
CP442_RS03215	700149..701099	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
CP442_RS03220	701340..701924	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
CP442_RS03225	701926..702636	+	711	Protein_626	transposase	-
CP442_RS03230	702639..703109	+	471	WP_003898523.1	hypothetical protein	-
CP442_RS03235	703154..703399	+	246	WP_070891125.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP442_RS03240	703396..703797	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP442_RS21450	704185..704352	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
CP442_RS21455	704385..704582	-	198	WP_003403191.1	hypothetical protein	-
CP442_RS03260	704662..705819	-	1158	WP_003898524.1	hypothetical protein	-
CP442_RS03265	705871..706092	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
CP442_RS03270	706234..706839	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T82462 WP_003403187.1 NZ_CP023586:703396-703797 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T82462 NZ_CP023586:703396-703797 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8841.17 Da
• Isoelectric Point: 10.3486

>AT82462 WP_070891125.1 NZ_CP023586:703154-703399 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLVRLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT82462 NZ_CP023586:703154-703399 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGTCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6