Detailed information of TA system TA082461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697064..697710
• Replicon: chromosome
• Accession: NZ_CP023586
• Organism: Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: CP442_RS03185
• Protein ID: WP_003403137.1
• Coordinates: 697064..697477 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: CP442_RS03190
• Protein ID: WP_003403139.1
• Coordinates: 697474..697710 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP442_RS03165	693147..694697	+	1551	WP_003905349.1	MCE family protein	-
CP442_RS03170	694749..695141	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
CP442_RS03175	695138..695395	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
CP442_RS03180	695578..696813	-	1236	WP_003403128.1	ATP-binding protein	-
CP442_RS03185	697064..697477	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP442_RS03190	697474..697710	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CP442_RS03195	697814..698320	-	507	WP_003900973.1	two component system sensor kinase HK1	-
CP442_RS03200	698434..698964	-	531	WP_003917320.1	two component system sensor kinase HK2	-
CP442_RS03205	698948..699643	-	696	WP_003918099.1	two-component system response regulator TcrA	-
CP442_RS03210	699766..700077	+	312	WP_003403164.1	hypothetical protein	-
CP442_RS03215	700149..701099	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
CP442_RS03220	701340..701924	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
CP442_RS03225	701926..702636	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T82461 WP_003403137.1 NZ_CP023586:c697477-697064 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T82461 NZ_CP023586:c697477-697064 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT82461 WP_003403139.1 NZ_CP023586:c697710-697474 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT82461 NZ_CP023586:c697710-697474 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89