Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 694749..695395 Replicon chromosome
Accession NZ_CP023586
Organism Mycobacterium tuberculosis strain MDRMA2491

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag CP442_RS03170 Protein ID WP_003403122.1
Coordinates 694749..695141 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag CP442_RS03175 Protein ID WP_003403125.1
Coordinates 695138..695395 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP442_RS03155 690411..691937 + 1527 WP_003403112.1 virulence factor Mce family protein -
CP442_RS03160 692000..693142 + 1143 WP_003911253.1 virulence factor Mce family protein -
CP442_RS03165 693147..694697 + 1551 WP_003905349.1 MCE family protein -
CP442_RS03170 694749..695141 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
CP442_RS03175 695138..695395 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
CP442_RS03180 695578..696813 - 1236 WP_003403128.1 ATP-binding protein -
CP442_RS03185 697064..697477 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
CP442_RS03190 697474..697710 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CP442_RS03195 697814..698320 - 507 WP_003900973.1 two component system sensor kinase HK1 -
CP442_RS03200 698434..698964 - 531 WP_003917320.1 two component system sensor kinase HK2 -
CP442_RS03205 698948..699643 - 696 WP_003918099.1 two-component system response regulator TcrA -
CP442_RS03210 699766..700077 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T82460 WP_003403122.1 NZ_CP023586:c695141-694749 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T82460 NZ_CP023586:c695141-694749 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT82460 WP_003403125.1 NZ_CP023586:c695395-695138 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT82460 NZ_CP023586:c695395-695138 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References