Detailed information of TA system TA082449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707326..3708000
• Replicon: chromosome
• Accession: NZ_CP023585
• Organism: Mycobacterium tuberculosis strain MDRDM1098

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP441_RS18175
• Protein ID: WP_003417282.1
• Coordinates: 3707326..3707754 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP441_RS18180
• Protein ID: WP_003417286.1
• Coordinates: 3707758..3708000 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP441_RS18150	3703148..3703549	-	402	WP_003417264.1	cytidine deaminase	-
CP441_RS18155	3703690..3704124	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP441_RS18160	3704121..3704555	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP441_RS18165	3704684..3706456	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP441_RS18170	3706456..3707247	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP441_RS18175	3707326..3707754	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP441_RS18180	3707758..3708000	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP441_RS18185	3708122..3708736	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP441_RS18190	3708733..3709398	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP441_RS18195	3709399..3709932	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP441_RS21955	3709929..3710081	-	153	WP_003916129.1	hypothetical protein	-
CP441_RS18205	3710117..3711378	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T82449 WP_003417282.1 NZ_CP023585:c3707754-3707326 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T82449 NZ_CP023585:c3707754-3707326 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT82449 WP_003417286.1 NZ_CP023585:c3708000-3707758 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT82449 NZ_CP023585:c3708000-3707758 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59