Detailed information of TA system TA082446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3177240..3177788
• Replicon: chromosome
• Accession: NZ_CP023585
• Organism: Mycobacterium tuberculosis strain MDRDM1098

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: CP441_RS15660
• Protein ID: WP_003414602.1
• Coordinates: 3177525..3177788 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: CP441_RS15655
• Protein ID: WP_003414599.1
• Coordinates: 3177240..3177521 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP441_RS15630	3172863..3173720	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
CP441_RS15635	3173762..3174346	-	585	WP_003899514.1	DUF1707 domain-containing protein	-
CP441_RS15640	3174450..3174698	+	249	WP_003913411.1	antitoxin VapB23	-
CP441_RS15645	3174695..3175075	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
CP441_RS15650	3175157..3176968	-	1812	WP_003906921.1	penicillin-binding protein	-
CP441_RS15655	3177240..3177521	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
CP441_RS15660	3177525..3177788	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP441_RS15665	3178161..3179015	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
CP441_RS15670	3179071..3180234	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
CP441_RS15675	3180251..3181465	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
CP441_RS15680	3181473..3182714	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T82446 WP_003414602.1 NZ_CP023585:3177525-3177788 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T82446 NZ_CP023585:3177525-3177788 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT82446 WP_003414599.1 NZ_CP023585:3177240-3177521 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT82446 NZ_CP023585:3177240-3177521 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8