Detailed information of TA system TA082374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2854031..2854671
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: CP440_RS13860
• Protein ID: WP_003412970.1
• Coordinates: 2854031..2854450 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: CP440_RS13865
• Protein ID: WP_003412975.1
• Coordinates: 2854447..2854671 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS13830	2849616..2850338	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
CP440_RS13840	2850855..2851082	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
CP440_RS13845	2851079..2851480	+	402	WP_003412963.1	PIN domain-containing protein	-
CP440_RS13850	2851515..2852435	-	921	WP_003412965.1	restriction endonuclease	-
CP440_RS21875	2852776..2853021	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CP440_RS13855	2853080..2854030	+	951	WP_003911916.1	Lsr2 family protein	-
CP440_RS13860	2854031..2854450	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP440_RS13865	2854447..2854671	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
CP440_RS13870	2854702..2857545	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
CP440_RS13875	2857617..2858018	-	402	WP_003412981.1	hypothetical protein	-
CP440_RS13880	2858018..2858488	-	471	WP_003899364.1	transcription antitermination factor NusB	-
CP440_RS13885	2858491..2859054	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T82374 WP_003412970.1 NZ_CP023584:c2854450-2854031 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T82374 NZ_CP023584:c2854450-2854031 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT82374 WP_003412975.1 NZ_CP023584:c2854671-2854447 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT82374 NZ_CP023584:c2854671-2854447 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09