Detailed information of TA system TA082362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194466..2195169
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CP440_RS10560
• Protein ID: WP_003409778.1
• Coordinates: 2194466..2194795 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CP440_RS10565
• Protein ID: WP_003409780.1
• Coordinates: 2194792..2195169 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS10540	2190849..2191919	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CP440_RS10545	2191916..2192431	+	516	WP_003409718.1	flavin reductase family protein	-
CP440_RS10550	2192428..2193489	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CP440_RS10555	2193486..2194256	+	771	WP_003409775.1	SDR family oxidoreductase	-
CP440_RS10560	2194466..2194795	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CP440_RS10565	2194792..2195169	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CP440_RS10570	2195166..2195756	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CP440_RS10575	2195811..2197175	+	1365	WP_096865254.1	HNH endonuclease	-
CP440_RS10580	2197330..2197782	-	453	WP_096865491.1	hypothetical protein	-
CP440_RS10585	2197846..2198247	+	402	WP_003409869.1	hypothetical protein	-
CP440_RS10590	2198240..2198422	-	183	WP_003409870.1	hypothetical protein	-
CP440_RS10595	2198536..2198886	-	351	WP_003899096.1	hypothetical protein	-
CP440_RS10600	2198897..2199799	-	903	WP_003899097.1	hypothetical protein	-
CP440_RS10605	2199820..2200011	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T82362 WP_003409778.1 NZ_CP023584:c2194795-2194466 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T82362 NZ_CP023584:c2194795-2194466 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT82362 WP_003409780.1 NZ_CP023584:c2195169-2194792 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT82362 NZ_CP023584:c2195169-2194792 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8