Detailed information of TA system TA082354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1384123..1384811
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: CP440_RS06795
• Protein ID: WP_003406304.1
• Coordinates: 1384380..1384811 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: CP440_RS06790
• Protein ID: WP_003406302.1
• Coordinates: 1384123..1384383 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS06770	1379700..1380524	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
CP440_RS06775	1380529..1381710	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
CP440_RS06780	1381787..1382887	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
CP440_RS06785	1383058..1384047	+	990	WP_003406301.1	malate dehydrogenase	-
CP440_RS06790	1384123..1384383	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
CP440_RS06795	1384380..1384811	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP440_RS06800	1384834..1386522	-	1689	WP_003910308.1	PE family protein	-
CP440_RS06810	1386702..1387562	+	861	WP_003900301.1	hypothetical protein	-
CP440_RS06815	1387643..1388473	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
CP440_RS06820	1388530..1388823	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
CP440_RS06825	1388820..1389089	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T82354 WP_003406304.1 NZ_CP023584:1384380-1384811 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T82354 NZ_CP023584:1384380-1384811 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT82354 WP_003406302.1 NZ_CP023584:1384123-1384383 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT82354 NZ_CP023584:1384123-1384383 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6