Detailed information of TA system TA082353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239257..1239825
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP440_RS06100
• Protein ID: WP_003405865.1
• Coordinates: 1239451..1239825 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP440_RS06095
• Protein ID: WP_003405863.1
• Coordinates: 1239257..1239454 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS06075	1235298..1235936	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP440_RS06080	1236026..1237033	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP440_RS06085	1237050..1238033	-	984	WP_003898731.1	hypothetical protein	-
CP440_RS06090	1238096..1239169	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP440_RS06095	1239257..1239454	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP440_RS06100	1239451..1239825	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP440_RS06105	1240028..1240726	+	699	WP_003898733.1	hypothetical protein	-
CP440_RS06110	1240844..1241029	+	186	WP_003901093.1	hypothetical protein	-
CP440_RS06115	1240956..1241231	-	276	WP_003405867.1	hypothetical protein	-
CP440_RS06120	1241474..1241797	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP440_RS06125	1241812..1242522	-	711	WP_003911399.1	hypothetical protein	-
CP440_RS21750	1242544..1242753	+	210	WP_003911400.1	hypothetical protein	-
CP440_RS06130	1242705..1243412	-	708	Protein_1164	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T82353 WP_003405865.1 NZ_CP023584:1239451-1239825 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T82353 NZ_CP023584:1239451-1239825 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT82353 WP_003405863.1 NZ_CP023584:1239257-1239454 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT82353 NZ_CP023584:1239257-1239454 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07