Detailed information of TA system TA082349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014528..1015147
• Replicon: chromosome
• Accession: NZ_CP023584
• Organism: Mycobacterium tuberculosis strain MDRDM627

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CP440_RS04955
• Protein ID: WP_003404726.1
• Coordinates: 1014713..1015147 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CP440_RS04950
• Protein ID: WP_003898641.1
• Coordinates: 1014528..1014707 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP440_RS04940	1010001..1011581	+	1581	WP_003909313.1	serine hydrolase	-
CP440_RS04945	1011578..1013971	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CP440_RS21710	1014244..1014402	+	159	WP_003404720.1	hypothetical protein	-
CP440_RS04950	1014528..1014707	+	180	WP_003898641.1	antitoxin	Antitoxin
CP440_RS04955	1014713..1015147	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CP440_RS04960	1015245..1016018	+	774	WP_003404735.1	VOC family protein	-
CP440_RS04965	1016083..1016532	+	450	WP_003404738.1	hypothetical protein	-
CP440_RS04970	1016667..1016897	-	231	WP_003898642.1	hypothetical protein	-
CP440_RS04975	1017064..1018572	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CP440_RS04980	1018574..1019812	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T82349 WP_003404726.1 NZ_CP023584:1014713-1015147 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T82349 NZ_CP023584:1014713-1015147 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT82349 WP_003898641.1 NZ_CP023584:1014528-1014707 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT82349 NZ_CP023584:1014528-1014707 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5