Detailed information of TA system TA082303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2203513..2204057
• Replicon: chromosome
• Accession: NZ_CP023583
• Organism: Mycobacterium tuberculosis strain MDRDM260

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TLU9
• Locus tag: CP439_RS10645
• Protein ID: WP_003409896.1
• Coordinates: 2203513..2203809 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: CP439_RS10650
• Protein ID: WP_070892843.1
• Coordinates: 2203806..2204057 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP439_RS10590	2198546..2198896	-	351	WP_003899096.1	hypothetical protein	-
CP439_RS10595	2198907..2199809	-	903	WP_003899097.1	hypothetical protein	-
CP439_RS10600	2199830..2200021	-	192	WP_003409876.1	hypothetical protein	-
CP439_RS10605	2200022..2200318	-	297	WP_003409877.1	hypothetical protein	-
CP439_RS10610	2200558..2200773	+	216	WP_003409878.1	antitoxin	-
CP439_RS10615	2200770..2201081	+	312	WP_003409881.1	type II toxin-antitoxin system VapC family toxin	-
CP439_RS21810	2201055..2201576	-	522	WP_003904745.1	hypothetical protein	-
CP439_RS10625	2201551..2201928	+	378	WP_010886136.1	type II toxin-antitoxin system toxin HigB	-
CP439_RS10630	2201970..2202419	+	450	WP_003409886.1	type II toxin-antitoxin system antitoxin HigA	-
CP439_RS10635	2202416..2202961	+	546	WP_003409891.1	SecB-like chaperone	-
CP439_RS10640	2202850..2203464	-	615	WP_003901296.1	hypothetical protein	-
CP439_RS10645	2203513..2203809	-	297	WP_003409896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP439_RS10650	2203806..2204057	-	252	WP_070892843.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CP439_RS10655	2204044..2204538	+	495	WP_003899099.1	hypothetical protein	-
CP439_RS10660	2204698..2205105	-	408	WP_003409913.1	type II toxin-antitoxin system VapC family toxin	-
CP439_RS10665	2205109..2205381	-	273	WP_003899100.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CP439_RS10670	2205414..2206634	-	1221	WP_003409919.1	TetR family transcriptional regulator Mce3R	-
CP439_RS10685	2207533..2208330	+	798	WP_003899101.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.64 Da
• Isoelectric Point: 6.8604

>T82303 WP_003409896.1 NZ_CP023583:c2203809-2203513 [Mycobacterium tuberculosis]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL

Nucleotide

• Length: 297 bp

>T82303 NZ_CP023583:c2203809-2203513 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9221.20 Da
• Isoelectric Point: 7.0150

>AT82303 WP_070892843.1 NZ_CP023583:c2204057-2203806 [Mycobacterium tuberculosis]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSATEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR

Nucleotide

• Length: 252 bp

>AT82303 NZ_CP023583:c2204057-2203806 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCACTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCAGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TLU9