Detailed information of TA system TA082274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 444170..445181
• Replicon: chromosome
• Accession: NZ_CP023583
• Organism: Mycobacterium tuberculosis strain MDRDM260

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: CP439_RS01955
• Protein ID: WP_003401859.1
• Coordinates: 444170..444763 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: CP439_RS01960
• Protein ID: WP_003401860.1
• Coordinates: 444792..445181 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP439_RS01935	439819..441201	+	1383	WP_003898415.1	magnesium transporter	-
CP439_RS01940	441213..442247	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
CP439_RS01945	442343..443026	+	684	WP_003401857.1	VTT domain-containing protein	-
CP439_RS01950	443015..444145	-	1131	WP_003401858.1	hypothetical protein	-
CP439_RS01955	444170..444763	-	594	WP_003401859.1	zeta toxin family protein	Toxin
CP439_RS01960	444792..445181	-	390	WP_003401860.1	hypothetical protein	Antitoxin
CP439_RS01965	445262..446473	-	1212	WP_003906353.1	VWA domain-containing protein	-
CP439_RS01970	446479..447081	-	603	WP_003898417.1	SRPBCC family protein	-
CP439_RS01975	447095..447991	-	897	WP_003898418.1	MoxR family ATPase	-
CP439_RS01980	447988..448581	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
CP439_RS01985	448578..449333	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T82274 WP_003401859.1 NZ_CP023583:c444763-444170 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T82274 NZ_CP023583:c444763-444170 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT82274 WP_003401860.1 NZ_CP023583:c445181-444792 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT82274 NZ_CP023583:c445181-444792 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0