Detailed information of TA system TA082272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363432..364003
• Replicon: chromosome
• Accession: NZ_CP023583
• Organism: Mycobacterium tuberculosis strain MDRDM260

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: CP439_RS01605
• Protein ID: WP_003401560.1
• Coordinates: 363432..363734 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: CP439_RS01610
• Protein ID: WP_003401563.1
• Coordinates: 363782..364003 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP439_RS01585	358937..359740	-	804	WP_003401540.1	hypothetical protein	-
CP439_RS01590	359750..361147	-	1398	WP_003401544.1	sulfatase	-
CP439_RS01595	361326..363065	+	1740	WP_031658820.1	PE family protein	-
CP439_RS01600	363208..363435	+	228	WP_031658821.1	ribbon-helix-helix protein, CopG family	-
CP439_RS01605	363432..363734	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
CP439_RS01610	363782..364003	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
CP439_RS01615	364000..364425	+	426	WP_003401566.1	PIN domain nuclease	-
CP439_RS01620	364561..365193	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
CP439_RS01625	365190..366098	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T82272 WP_003401560.1 NZ_CP023583:363432-363734 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T82272 NZ_CP023583:363432-363734 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT82272 WP_003401563.1 NZ_CP023583:363782-364003 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT82272 NZ_CP023583:363782-364003 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48