Detailed information of TA system TA082231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239305..1239873
• Replicon: chromosome
• Accession: NZ_CP023582
• Organism: Mycobacterium tuberculosis strain LN3756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP438_RS06085
• Protein ID: WP_003405865.1
• Coordinates: 1239499..1239873 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP438_RS06080
• Protein ID: WP_003405863.1
• Coordinates: 1239305..1239502 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP438_RS06060	1235346..1235984	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP438_RS06065	1236074..1237081	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP438_RS06070	1237098..1238081	-	984	WP_003898731.1	hypothetical protein	-
CP438_RS06075	1238144..1239217	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP438_RS06080	1239305..1239502	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP438_RS06085	1239499..1239873	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP438_RS06090	1240076..1240774	+	699	WP_003898733.1	hypothetical protein	-
CP438_RS06095	1240892..1241077	+	186	WP_003901093.1	hypothetical protein	-
CP438_RS06100	1241004..1241279	-	276	WP_003405867.1	hypothetical protein	-
CP438_RS06105	1241522..1241845	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP438_RS06110	1241860..1242570	-	711	WP_003911399.1	hypothetical protein	-
CP438_RS21745	1242592..1242801	+	210	WP_003911400.1	hypothetical protein	-
CP438_RS06115	1242753..1243460	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T82231 WP_003405865.1 NZ_CP023582:1239499-1239873 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T82231 NZ_CP023582:1239499-1239873 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT82231 WP_003405863.1 NZ_CP023582:1239305-1239502 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT82231 NZ_CP023582:1239305-1239502 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07