Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 697087..697733 Replicon chromosome
Accession NZ_CP023582
Organism Mycobacterium tuberculosis strain LN3756

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag CP438_RS03185 Protein ID WP_003403137.1
Coordinates 697087..697500 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag CP438_RS03190 Protein ID WP_003403139.1
Coordinates 697497..697733 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP438_RS03165 693170..694720 + 1551 WP_003403119.1 MCE family protein -
CP438_RS03170 694772..695164 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
CP438_RS03175 695161..695418 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
CP438_RS03180 695601..696836 - 1236 WP_003403128.1 ATP-binding protein -
CP438_RS03185 697087..697500 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
CP438_RS03190 697497..697733 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CP438_RS03195 697837..698343 - 507 WP_003900973.1 two component system sensor kinase HK1 -
CP438_RS03200 698457..698987 - 531 WP_003917320.1 two component system sensor kinase HK2 -
CP438_RS03205 698971..699666 - 696 WP_003918099.1 two-component system response regulator TcrA -
CP438_RS03210 699789..700100 + 312 WP_003403164.1 hypothetical protein -
CP438_RS03215 700172..701122 + 951 WP_003900184.1 DUF1259 domain-containing protein -
CP438_RS03220 701363..701947 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
CP438_RS03225 701949..702659 + 711 Protein_626 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T82217 WP_003403137.1 NZ_CP023582:c697500-697087 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T82217 NZ_CP023582:c697500-697087 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT82217 WP_003403139.1 NZ_CP023582:c697733-697497 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT82217 NZ_CP023582:c697733-697497 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References