Detailed information of TA system TA082003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2234069..2234655
• Replicon: chromosome
• Accession: NZ_CP023578
• Organism: Mycobacterium tuberculosis strain LE486

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CP434_RS10845
• Protein ID: WP_070892640.1
• Coordinates: 2234069..2234413 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: CP434_RS10850
• Protein ID: WP_003410014.1
• Coordinates: 2234407..2234655 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP434_RS10805	2229775..2230374	+	600	WP_003409989.1	L-lysine exporter	-
CP434_RS10810	2230790..2231218	+	429	WP_003906713.1	cellulose-binding protein	-
CP434_RS10815	2231444..2231983	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
CP434_RS10825	2232503..2233063	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
CP434_RS10830	2233060..2233401	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
CP434_RS10835	2233487..2233744	+	258	WP_003410006.1	hypothetical protein	-
CP434_RS10840	2233645..2233980	-	336	WP_003410009.1	dehydrogenase	-
CP434_RS10845	2234069..2234413	-	345	WP_070892640.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
CP434_RS10850	2234407..2234655	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
CP434_RS10855	2234755..2237070	-	2316	WP_096865255.1	cation transporter ATPase CptG	-
CP434_RS10860	2237067..2237339	-	273	WP_003410017.1	DUF1490 family protein	-
CP434_RS10865	2237392..2237748	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
CP434_RS10870	2237905..2238672	+	768	WP_003410019.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12214.12 Da
• Isoelectric Point: 9.8887

>T82003 WP_070892640.1 NZ_CP023578:c2234413-2234069 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQADPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T82003 NZ_CP023578:c2234413-2234069 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGGCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT82003 WP_003410014.1 NZ_CP023578:c2234655-2234407 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT82003 NZ_CP023578:c2234655-2234407 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAW9