Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 696941..697587 Replicon chromosome
Accession NZ_CP023578
Organism Mycobacterium tuberculosis strain LE486

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag CP434_RS03190 Protein ID WP_003403137.1
Coordinates 696941..697354 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag CP434_RS03195 Protein ID WP_003403139.1
Coordinates 697351..697587 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP434_RS03170 693024..694574 + 1551 WP_003403119.1 MCE family protein -
CP434_RS03175 694626..695018 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
CP434_RS03180 695015..695272 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
CP434_RS03185 695455..696690 - 1236 WP_003403128.1 ATP-binding protein -
CP434_RS03190 696941..697354 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
CP434_RS03195 697351..697587 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CP434_RS03200 697691..698197 - 507 WP_003900973.1 two component system sensor kinase HK1 -
CP434_RS03205 698311..698841 - 531 WP_003917320.1 two component system sensor kinase HK2 -
CP434_RS03210 698825..699520 - 696 WP_003918099.1 two-component system response regulator TcrA -
CP434_RS03215 699643..699954 + 312 WP_003403164.1 hypothetical protein -
CP434_RS03220 700026..700976 + 951 WP_003900184.1 DUF1259 domain-containing protein -
CP434_RS03225 701217..701801 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
CP434_RS03230 701803..702513 + 711 Protein_626 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T81975 WP_003403137.1 NZ_CP023578:c697354-696941 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T81975 NZ_CP023578:c697354-696941 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT81975 WP_003403139.1 NZ_CP023578:c697587-697351 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT81975 NZ_CP023578:c697587-697351 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References