81972

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 640014..640690 Replicon chromosome
Accession NZ_CP023578
Organism Mycobacterium tuberculosis strain LE486

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag CP434_RS02930 Protein ID WP_003898500.1
Coordinates 640014..640427 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag CP434_RS02935 Protein ID WP_003402915.1
Coordinates 640424..640690 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP434_RS02900 635359..635661 - 303 WP_003402896.1 DUF3349 domain-containing protein -
CP434_RS02905 635721..635999 - 279 WP_003402901.1 hypothetical protein -
CP434_RS02910 635996..637249 - 1254 WP_003402904.1 inorganic phosphate transporter family protein -
CP434_RS02915 637369..637755 - 387 WP_003402907.1 VOC family protein -
CP434_RS02920 637818..638702 - 885 WP_003402909.1 SDR family oxidoreductase -
CP434_RS02925 638798..639700 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
CP434_RS02930 640014..640427 - 414 WP_003898500.1 PIN domain-containing protein Toxin
CP434_RS02935 640424..640690 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
CP434_RS02940 640882..642597 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
CP434_RS02945 642675..644279 + 1605 WP_003402920.1 amidohydrolase -
CP434_RS02950 644276..645256 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T81972 WP_003898500.1 NZ_CP023578:c640427-640014 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T81972 NZ_CP023578:c640427-640014 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT81972 WP_003402915.1 NZ_CP023578:c640690-640424 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT81972 NZ_CP023578:c640690-640424 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References